OBJECTIVE: To investigate the role of miR-145 silencing in the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF). B-cell lymphoma-2 (BcI-2), BCL2-Associated X(bax) and caspase-3 in avascular necrosis of femoral head (A NFH). MATERIALS AND METHODS: A total of 12 healthy wild-types (the control group) and 12 miR-145 knock-out (miR-145-/-) (the experimental group) adult New Zealand white rabbits were selected to construct ANFH model with steroid. Four weeks later, immunohistochemistry, qRT-PCR and Western blot were performed to measure the VEGF, bFGF, BcI-2, bax, caspase-3, beta-catenin as well as c-Myc expression. Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining analysis was used to detect the apoptosis of bone cells in each group. RESULTS: Compared with the control group, the expression of VEGF, bFGF, BcI-2, beta-catenin and c-Myc in the miR-145-/- group raised (p < 0.05). Moreover, the expression level of bax and caspase-3 significantly decreased in the miR-145-/- group (p<0.05). TUNEL staining showed decreased apoptosis in the miR-145-/- group. CONCLUSIONS: MiR-145 silencing promotes bone repair of ANFH via upregulating VEGF, bFGF and inhibiting the bone cells apoptosis through Wnt/beta-catenin pathway.
第一作者机构：[1]Hebei Univ Engn, Affiliated Hosp, Dept Orthoped, Handan, Hebei, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Tian Z. -J.,Liu B. -Y.,Zhang Y. -T.,et al.MiR-145 silencing promotes steroid-induced avascular necrosis of the femoral head repair via upregulating VEGF[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2017,21(17):3763-3769.
APA：
Tian, Z. -J.,Liu, B. -Y.,Zhang, Y. -T.,Chen, X. -Z.,Qiao, G. -Y....&Zhilin Ma.(2017).MiR-145 silencing promotes steroid-induced avascular necrosis of the femoral head repair via upregulating VEGF.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,21,(17)
MLA：
Tian, Z. -J.,et al."MiR-145 silencing promotes steroid-induced avascular necrosis of the femoral head repair via upregulating VEGF".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 21..17(2017):3763-3769
