机构：[1]Third Mil Med Univ, Southwest Hosp, Dept Oncol, Chongqing 400038, Peoples R China[2]Third Mil Med Univ, Southwest Hosp, Southwest Canc Ctr, Chongqing 400038, Peoples R China[3]Hebei Univ Chinese Med, Hebei Prov Hosp Tradit Chinese Med, Dept Radiotherapy, Shijiazhuang 050011, Hebei, Peoples R China[4]Hebei Univ Chinese Med, Hebei Prov Hosp Tradit Chinese Med, Dept Radiol, Shijiazhuang 050011, Hebei, Peoples R China
Glucocorticoids are effective to treat lymphoma and leukemia. Their effect in colon cancer remains far from clear. Here, we found that glucocorticoid receptor (GR) a protein level was dramatically lower in colon cancer than in lymphoma. Colon cell lines LoVo and HCT116 were GR alpha-rich and GR alpha was not detectable in HT29 or SW480. Dexamethasone significantly inhibited cell growth of GR alpha-rich cell lines and did not significantly affect GR alpha-negative cell lines. Dexamethasone induced apoptosis and increased chemosensitivity of GR alpha-rich cell lines. Knockdown of GR alpha significantly attenuated dexamethasone effects on cell growth, apoptosis and chemosensitivity. NF-kappa B p65 significantly correlated with GR alpha in colon cancer samples. Dexamethasone decreased NF-kappa B p65 activity. Knockdown of NF-kappa B p65 increased apoptosis. Our data demonstrate GRa protein level is dramatically lower in colon cancer than in lymphoma. Dexamethasone inhibits cell growth, induces apoptosis and enhances chemosensitivity in colon cancer, at least partly, via GR alpha and NF-kappa B.
基金：
Chongqing Science & Technology Commission grant [cstc2016jcyjA2109]
第一作者机构：[1]Third Mil Med Univ, Southwest Hosp, Dept Oncol, Chongqing 400038, Peoples R China[2]Third Mil Med Univ, Southwest Hosp, Southwest Canc Ctr, Chongqing 400038, Peoples R China[3]Hebei Univ Chinese Med, Hebei Prov Hosp Tradit Chinese Med, Dept Radiotherapy, Shijiazhuang 050011, Hebei, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Third Mil Med Univ, Southwest Hosp, Dept Oncol, Chongqing 400038, Peoples R China[2]Third Mil Med Univ, Southwest Hosp, Southwest Canc Ctr, Chongqing 400038, Peoples R China
推荐引用方式(GB/T 7714)：
He Jianming,Zhou Jinming,Yang Weiwen,et al.Dexamethasone affects cell growth/apoptosis/chemosensitivity of colon cancer via glucocorticoid receptor α/NF-κB[J].ONCOTARGET.2017,8(40):67670-67683.doi:10.18632/oncotarget.18802.
APA：
He, Jianming,Zhou, Jinming,Yang, Weiwen,Zhou, Qi,Liang, Xi...&Liang, Houjie.(2017).Dexamethasone affects cell growth/apoptosis/chemosensitivity of colon cancer via glucocorticoid receptor α/NF-κB.ONCOTARGET,8,(40)
MLA：
He, Jianming,et al."Dexamethasone affects cell growth/apoptosis/chemosensitivity of colon cancer via glucocorticoid receptor α/NF-κB".ONCOTARGET 8..40(2017):67670-67683
