A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement, mineralized function expression and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effect of Nd3+ and Sm3+ on the proliferation, differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels. The experimental results suggest that concentration, culture time and ion species are pivotal factors for switching the biological effects of rare earth ions from toxicity to activity, from damage to protection, or from down-regulation to up-regulation.
基金：
National Natural Science Foundation of China [20971034]; Natural Science Foundation of Hebei Province [B2009000161]; Ministry of Education of China [208018]; Returned Scholars of Hebei Province [207041]; Natural Science Foundation of Hebei University
第一作者机构：[1]Chemical Biology Key Laboratory of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang JinChao,Shang MiQin,Zhang DaWei,et al.Effects of Nd3+ and Sm3+ on the proliferation, differentiation and mineralization function of primary osteoblasts in vitro[J].CHINESE SCIENCE BULLETIN.2010,55(23):2505-2511.doi:10.1007/s11434-010-3153-0.
APA：
Zhang JinChao,Shang MiQin,Zhang DaWei,Li YaPing,Sun Jing&Chen Hang.(2010).Effects of Nd3+ and Sm3+ on the proliferation, differentiation and mineralization function of primary osteoblasts in vitro.CHINESE SCIENCE BULLETIN,55,(23)
MLA：
Zhang JinChao,et al."Effects of Nd3+ and Sm3+ on the proliferation, differentiation and mineralization function of primary osteoblasts in vitro".CHINESE SCIENCE BULLETIN 55..23(2010):2505-2511
