Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts
机构：[1]College of Chemistry and Environmental Science, Chemical Biology Laboratory, Hebei University, Baoding[2]Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding[3]B-Ultrasound Room, Affiliated Hospital of Hebei University, Baoding河北大学附属医院[4]Department of Stomatology, Affiliated Hospital of Hebei University, Baoding, PR China河北大学附属医院
The effects of water extract of Cajanus cajan (Linn.) Millsp. (Leguminosae) leaves (WECML) on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipocytic trans-differentiation of mouse primary osteoblasts (OBs) were studied. The results indicated that WECML promoted the proliferation of BMSCs and OBs at most concentrations. WECML promoted the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentrations of 0.1, 1, and 10 mu g/mL, but inhibited the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentration of 0.01 mu g/mL. WECML inhibited the adipogenic differentiation of BMSCs and adipocytic trans-differentiation of OBs at concentrations of 0.001, 0.1, 1, 10, and 100 mu g/mL, but had no effects at concentration of 0.01 mu g/mL. The results suggest that WECML has protective effects on bone and these protective effects may be mediated by decreasing adipocytic cell formation from BMSCs, which may promote the proliferation, differentiation, and mineralization function of OBs. The defined active ingredients in the WECML and the active mechanism need to be further studied.
基金：
Ministry of Education of China [208018]; Natural Science Foundation of Hebei University; Returned Scholars of Hebei Province [207041]
第一作者机构：[1]College of Chemistry and Environmental Science, Chemical Biology Laboratory, Hebei University, Baoding[2]Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding
通讯作者：
通讯机构：[1]College of Chemistry and Environmental Science, Chemical Biology Laboratory, Hebei University, Baoding[2]Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding
推荐引用方式(GB/T 7714)：
Zhang Jinchao,Liu Cuilian,Sun Jing,et al.Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts[J].PHARMACEUTICAL BIOLOGY.2010,48(1):89-95.doi:10.3109/13880200903046179.
APA：
Zhang, Jinchao,Liu, Cuilian,Sun, Jing,Liu, Dandan&Wang, Peng.(2010).Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts.PHARMACEUTICAL BIOLOGY,48,(1)
MLA：
Zhang, Jinchao,et al."Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts".PHARMACEUTICAL BIOLOGY 48..1(2010):89-95
