Effects of Cerium Oxide Nanoparticles on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells In Vitro
机构：[1]College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education,Hebei University, Baoding 071000, China[2]Department of Science and Technology, Affiliated Hospital of Hebei University, Baoding 071000, P. R. China河北大学附属医院
The effects of cerium oxide nanoparticles (nanoceria) on the proliferation, osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (BMSCs) were studied by employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MU), alkaline phosphatase (ALP) activity, collagen production, alizarin red-S (ARS) and oil red o stain assays. The results indicated that nanoceria increased the viability of BMSCs at all tested concentrations with evident dose dependence for 24 and 72 h. On day 14, nanoceria inhibited the osteogenic differentiation of BMSCs at all tested concentrations. On day 19 and 24, nanoceria inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations. On day 17, nanoceria inhibited the adipogenic differentiation of BMSCs at all tested concentrations. This suggests that the effects of nanoceria on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated. Both the concentration and culture time have significant influence on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs. These results will be helpful for rational applications of nanoceria in the future.
基金：
Supported by Chinese Natural Science Foundation project (No. 21271059), Research Fundfor the Doctoral Program of Higher Education of China(No. 20111301110004), Hebei Province “Hundred Talents Program” (BR2-202), Training Program for Innovative Research Team and Leading Talent in Hebei ProvinceUniversity (LJRC024).
第一作者机构：[1]College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education,Hebei University, Baoding 071000, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Qun,Ge Kun,Ren Huihui,et al.Effects of Cerium Oxide Nanoparticles on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells In Vitro[J].JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY.2015,15(9):6444-6451.doi:10.1166/jnn.2015.10709.
APA：
Zhang, Qun,Ge, Kun,Ren, Huihui,Zhang, Cuimiao&Zhang, Jinchao.(2015).Effects of Cerium Oxide Nanoparticles on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells In Vitro.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,15,(9)
MLA：
Zhang, Qun,et al."Effects of Cerium Oxide Nanoparticles on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells In Vitro".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 15..9(2015):6444-6451
