机构：[1]Pediatric Intensive Care Unit, Beijing Children's Hospital, Capital Medical University, Beijing, China首都医科大学附属北京儿童医院[2]Department of Pediatrics, Affiliated Hospital of Hebei University, Baoding, China河北大学附属医院[3]Key Laboratory of Major Diseases in Children, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China首都儿科研究所首都医科大学附属北京儿童医院[4]Youyang Hospital, People's Hospital of Chongqing Youyang County, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China重庆医科大学附属第一医院
Background The aim of this study was to analyze the microbiological characteristics of nasopharyngeal carriage Haemophilus influenzae isolates collected from children with respiratory infections in Beijing hospital and Youyang Hospital of China. Methods The serotypes of all isolates were determined using latex agglutinated antisera (a-f). The minimum inhibitory concentrations (MICs) of 11 antibiotics were determined using E-test strips. For the beta-lactamase-negative ampicillin-resistant (BLNAR) isolates, ftsI gene was sequenced based on fragments amplified by PCR. STs of H influenzae isolates were determined by multi-locus sequence typing. Results The overall carriage rate of H influenzae in the study population was 9.1% (362/3984). One hundred and ninety H influenzae isolates which were selected in our study were non-typeable (NTHi) and 44 (23.2%) of them were positive for beta-lactamase. All isolates were susceptible to ceftriaxone and levofloxacin. Susceptibility rates to erythromycin and sulfamethoxazole-trimethoprim in Beijing were significantly higher than Youyang (P < .05). Thirty-six BLNAR isolates were identified. The MLST analysis showed 108 STs in 190 isolates, the most common of which were ST408 (11, 5.8%), ST914 (10, 5.3%), ST57 (9, 4.7%), and ST834 (6, 3.2%). Twelve STs were detected in both of the study sites, which covered 63 isolates. Conclusions All isolates in the present study were NTHi, which suggested widespread of this type in China. The BLNAR isolates were detected more frequently than before. Because high genetic diversity of NTHi isolates of H influenzae exists worldwide, it is important to continuously monitor these bacteria in the future.
基金：
This study was supported by a national
clinical center project from the Ministry
of Science and Technology of the People's
Republic of China (2013BAI09B11)
and the Beijing Talents Fund
(2015000021469G209).
第一作者机构：[1]Pediatric Intensive Care Unit, Beijing Children's Hospital, Capital Medical University, Beijing, China[2]Department of Pediatrics, Affiliated Hospital of Hebei University, Baoding, China
通讯作者：
通讯机构：[1]Pediatric Intensive Care Unit, Beijing Children's Hospital, Capital Medical University, Beijing, China[3]Key Laboratory of Major Diseases in Children, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China[*1]Key Laboratory of Major Diseases in Children, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 56 Nanlishi Road, Xicheng District, Beijing 100045 China.[*2]Pediatric Intensive Care Unit, Beijing Children's Hospital, Capital Medical University, 56 Nanlishi Road, Xicheng District, Beijing 100045, China.
推荐引用方式(GB/T 7714)：
Dong Qiaoli,Shi Wei,Cheng Xiaoping,et al.Widespread of non-typeable Haemophilus influenzae with high genetic diversity after two decades use of Hib vaccine in China[J].JOURNAL OF CLINICAL LABORATORY ANALYSIS.2020,34(4):doi:10.1002/jcla.23145.
APA：
Dong, Qiaoli,Shi, Wei,Cheng, Xiaoping,Chen, Changhui,Meng, Qinghong...&Qian, Suyun.(2020).Widespread of non-typeable Haemophilus influenzae with high genetic diversity after two decades use of Hib vaccine in China.JOURNAL OF CLINICAL LABORATORY ANALYSIS,34,(4)
MLA：
Dong, Qiaoli,et al."Widespread of non-typeable Haemophilus influenzae with high genetic diversity after two decades use of Hib vaccine in China".JOURNAL OF CLINICAL LABORATORY ANALYSIS 34..4(2020)
