The objective of this study was to investigate the early diagnosis effect and clinical predictive value of serum amyloid A and C reactive protein in children with acute upper respiratory tract infection. A total of 324 children with acute upper respiratory tract infection (ARTI) who were treated in our hospital from October 2020 to April 2022 were selected as the research subjects. The variation of C-reactive protein concentration was tested by sphericity hypothesis, and the Greenhouse-Geisser method was used for in-depth analysis. The Receiver-Operating Characteristic (ROC) curve was drawn to evaluate the early predictive value of serum amyloid A and C reactive protein in children with acute upper respiratory tract. The concentrations of serum amyloid A and C reactive protein in children with ARTI changed significantly, with the increase of time, the concentration decreased, and the difference was statistically significant (P <0.05). The concentrations of amyloid A and C-reactive protein were significantly different, and the difference was statistically significant (P <0.05). Finally, the predictive effect of serum amyloid A and C reactive protein changes in children with ARTI was analyzed. From the ROC curve, it can be known that for both viral and bacterial upper respiratory tract infections, serum amyloid A and C reactive protein Proteins all have higher area under the line, and the ratio of the two is larger than the area under the line, both above 0.8. The serum amyloid A and C-reactive protein in the blood routine test of children with ARTI showed a changing trend. It was concluded that the changes of serum amyloid A and C-reactive protein can be used for early prediction of children with ARTI. It is of great value to improve the clinical treatment of ARTI.