The emergence and establishment of nations greatly emphasize the significance of developing and producing innovative therapeutic medications that exhibit exceptional effectiveness in treating nephropathy. The investigation evaluated the impact of Ag nanoparticles (AgNPs) produced via an environmentally friendly technique utilizing a water-based extract of Scutellaria baicalensis on nephroprotective potentials. The silver nanoparticles synthesis was achieved by combining Scutellaria baicalensis with Ag nitrate solution. The characteristics were determined using FT-IR, EDX, UV-Vis, and FE-SEM techniques. The TEM and FE-SEM images obtained from silver nanoparticles under the designated optimal conditions illustrate the spherical nanoparticles formation. The resulting particles exhibited a size distribution between 10 and 50 nm. Diabetes was induced in one hundred mature male mice through the intraperitoneal administration of 60 mg/kg of streptozotocin (STZ). Following this, the animals were randomly divided into five groups. The control animals were administered with regular saline, whereas the experimental groups were administered with glibenclamide, along with 25 and 100 mu g/kg of Ag NPs. A particular group was assigned as the control group for the diabetic subjects who received treatment. At the end of the research, the serum concentrations of glucose, urea, and creatinine in the specimens were evaluated. After tissue processing, the kidneys were carefully sectioned into 5 mu m slices and then stained PAS for further stereological examination. The AgNPs groups showed a notable reduction (p < 0.05) in blood glucose and urea levels in comparison to the diabetic group that did not receive treatment. Following AgNPs-100 administration, a notable reduction (p < 0.05) was noted in the kidney volume, kidney weight, and the length of kidney structures. According to the results, silver nanoparticles obtained from Scutellaria baicalensis demonstrated efficient control over blood glucose and urea levels, while also offering protection against kidney damage in diabetic mice induced by STZ. The study suggests the use of environmentally-friendly silver NPs as a promising treatment for kidney protection and diabetes in emerging countries.