Gestational Diabetes Mellitus (GDM) is the utmost common metabolic disorder of pregnancy, related with numerous perinatal complications. Recently, several researches have shown the valuable effect of exercise and medical nutrition therapies on weight control and glycemic control in GDM-affected peoples. In the present investigation, we established a rat model of gestational diabetes mellitus (GDM) rats by utilizing streptozotocin (STZ) with Murraya koenigii (M. koenigii) extract treated Au-PLGA nanoformulated system. An aggregate of thirty rats was confined together with male rats for pregnancy, out of which twenty-seven pregnant rats were acquired and weighed. The pregnant animals were similarly randomized into control gathering, gestational diabetes mellitus gathering and intercession gathering. After the treatment of M. koenigii leaf extract with Au-PLGA nanosystem, serum glucose and lipids levels of intercession amass were enhanced considerably. Antioxidant enzymes levels of gestational diabetes mellitus aggregate in pancreas and liver tissues were lower than alternate gatherings, and after treatment of M. koenigii leaf extract, cell reinforcement compounds levels in pancreas and liver tissues were equal to the control gathering. M. koenigii leaf extract, wealthy antioxidant agent substances, could maintain a strategic distance from the extreme expending of cell reinforcement chemicals, at that point, smothering the oxidative pressure and insulin opposition, and, along these lines, enhancing blood glucose level of gestational diabetes mellitus in rats.