The canonical Wnt pathway is vital to bone physiology by increasing bone mass through elevated osteoblast survival. Although investigated extensively in stem cells, its role in osteoblastic MC3T3-E1 cells has not been completely determined. To explore how this pathway is regulated by different conditions, we assessed the anti-apoptotic effects of substance P on the canonical Wnt pathway in MC3T3-E1 cells by treating cells with serum deprivation or serum starving with substance P, a neuropeptide demonstrated to promote bone growth and stimulate Wnt signaling. The results showed that serum deprivation both induced apoptosis and activated Wnt signal transduction while substance P further stimulated the Wnt pathway via the NK-1 receptor but protected the cells from apoptotic death. Fast-tracking of Wnt signaling by substance P was also noted. These results indicate that nutritional deprivation and substance P synergistically activated the canonical Wnt pathway, a finding that helps to reveal the role of Wnt signaling in bone physiology affected by nutritional deprivation and neuropeptide substance P.
第一作者机构：[1]Southern Med Univ, Dept Orthoped & Traumatol, Nanfang Hosp, Guangzhou 510515, Guangdong, Peoples R China[2]Huhhot First Hosp, Dept Orthopaed, Hohhot 010020, Inner Mongolia, Peoples R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Jianguo,Nie Jiping,Fu Su,et al.The fast track to canonical Wnt signaling in MC3T3-E1 cells protected by substance P against serum deprivation-induced apoptosis[J].CELL BIOLOGY INTERNATIONAL.2017,41(1):71-78.doi:10.1002/cbin.10676.
APA：
Yang, Jianguo,Nie, Jiping,Fu, Su,Liu, Song,Wu, Jianqun...&Yu, Bin.(2017).The fast track to canonical Wnt signaling in MC3T3-E1 cells protected by substance P against serum deprivation-induced apoptosis.CELL BIOLOGY INTERNATIONAL,41,(1)
MLA：
Yang, Jianguo,et al."The fast track to canonical Wnt signaling in MC3T3-E1 cells protected by substance P against serum deprivation-induced apoptosis".CELL BIOLOGY INTERNATIONAL 41..1(2017):71-78
