To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units; and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26 +, CD44 +, ALDH1 + and CD133 + cells co-existed with Lgr5 + cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated. (C) 2013 Elsevier B.V. All rights reserved.
基金：
Hebei University for Initiating Research Project of Importing Talented Teachers [2011-216]; Foundation of Hebei Education Department for Outstanding Youths [Y2012024]
第一作者机构：[1]Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China[*1]Hebei Univ, Sch Life Sci, 180 East Wusi Rd, Baoding 071002, Hebei Province, Peoples R China
推荐引用方式(GB/T 7714)：
Wu Chen,Xie Yuanyuan,Gao Feng,et al.Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers[J].GENE.2013,525(1):18-25.doi:10.1016/j.gene.2013.04.067.
APA：
Wu, Chen,Xie, Yuanyuan,Gao, Feng,Wang, Yanan,Guo, Yawei...&Fan, Wufang.(2013).Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers.GENE,525,(1)
MLA：
Wu, Chen,et al."Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers".GENE 525..1(2013):18-25
