BACKGROUND: The effects of a high protein diet on insulin secretion and glucose metabolism have been quite controversial. The aim of this study was to evaluate the effects of long-term isocaloric high animal protein intake on insulin secretion in diet-induced obese rats. RESULTS: After the experimental period (24 weeks), the high-fat diet-induced obese rats that were fed isocaloric high-protein diets (HP) had lower body weight gain (P < 0.01) and lower visceral fat (P < 0.05) than normal protein (NP) rats. Fasting plasma glucagon-like peptide-1 (GLP-1) was also reduced significantly (P < 0.05), as well as serum insulin levels at 5 min and 10 min by intravenous insulin releasing test. In addition, insulin mRNA and pancreatic duodenal homeodomain-1 (PDX-1), GLP-1 protein expression were both markedly lower in HP rats (P < 0.05), while PDX-1 mRNA in HP rats had no difference from NP rats. CONCLUSION: These results suggest that long-term isocaloric high animal protein intake reduces the acute insulin response in obese rats and the decrease of insulin is associated with both reduced weight gain and inhibition of PDX-1 expression. GLP-1 might be a negative feedback for the balance of energy metabolism secondary to changes of body weight and visceral fat. Copyright (C) 2012 Society of Chemical Industry
基金：
Natural Science Foundation of Shandong Province [Z2006C09]
第一作者机构：[1]Shandong Univ, Dept Endocrinol, Prov Hosp Affiliated, Jinan 250021, Shandong, Peoples R China
通讯作者：
通讯机构：[1]Shandong Univ, Dept Endocrinol, Prov Hosp Affiliated, Jinan 250021, Shandong, Peoples R China[*1]Shandong Univ, Dept Endocrinol, Prov Hosp Affiliated, 324 Jingwu Rd, Jinan 250021, Shandong, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Haiyan,Wang Yiling,Ma Lichuan,et al.Long-term high animal protein diet reduces body weight gain and insulin secretion in diet-induced obese rats[J].JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE.2012,92(13):2638-2643.doi:10.1002/jsfa.5679.
APA：
Chen, Haiyan,Wang, Yiling,Ma, Lichuan,Zhao, Jiajun,Li, Yinyin&Li, Minglong.(2012).Long-term high animal protein diet reduces body weight gain and insulin secretion in diet-induced obese rats.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,92,(13)
MLA：
Chen, Haiyan,et al."Long-term high animal protein diet reduces body weight gain and insulin secretion in diet-induced obese rats".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 92..13(2012):2638-2643
