机构：[1]School of Traditional Chinese Medicine, Beijing University of Chinese Medicine[2]Dongfang Hospital, Beijing University of Chinese Medicine[3]Graduate School, Shanxi University of Chinese Medicine[4]Beijing University of Chinese Medicine Libraries[5]Shanxi Modern Chinese Medicine Engineering Laboratory, Shanxi University of Chinese Medicine[6]National Cancerology Institute of Mexico, National Polytechnic University of Mexico
Objective:To investigate the effects of Ganjiang granule(GG) on cecal microflora and serum biochemical components in rats with constipation-predominant irritable bowel syndrome(IBS-C).Methods:Twenty-four Sprague-Dawley rats were randomly divided into four groups:control,model,GG,and probiotics.Rats in the model,GG,and probiotics groups received 3℃ tap water intragastrically;rats in the GG group were treated with GG;rats in the probiotics group were treated with probiotics.For all rats,enzyme-linked immunosorbent assay and colorimetry were used to assess serum biochemical components related to gastrointestinal function;16 S rRNA high-throughput sequencing was used to analyze the cecal microflora.Results:The serum level of 5-hydroxytryptamine(HT) was higher in the model group than in the control group(Z=-2.082,P=.037).The model group exhibited changes in cecal microflora:the relative abundances of Lactobacillus decreased(Z=-2.882,P=.004) and Dorea increased(t=-3.030,P=.023),compared with the control group.The GG and probiotics groups exhibited normal serum levels of 5-HT.The GG and probiotics groups exhibited improved serum levels of gastrin;the probiotics group exhibited an improved serum level of vasoactive intestinal peptide.Compared with the model group,The GG group exhibited greater relative abundance of Ruminococcus(Z=-2.402,P=.016);the probiotics group exhibited greater relative abundance of SMB53(Z=-2.823,P=.005) and lower relative abundances of Desulfovibrio(Z=-2.823,P=.005) and Facklamia(Z=-2.608,P=.009).Conclusion:The effects of GG on IBS-C may be related to regulation of the serum level of 5-HT,as well as elevated relative abundance of beneficial bacteria in cecal microflora.
基金：
supported by the National Natural Science Foundation of China (81473553,81573880 and 81873211)
基金编号：81473553,81573880 and 81873211
语种：
中文
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
通讯作者：
推荐引用方式(GB/T 7714)：
Lujiao Gao,Xin Niu,Tingli Niu,等.Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome[J].Journal of Traditional Chinese Medical Sciences.2020,7(02):148-153.
