机构：[1]School of Traditional Chinese Medicine, Beijing University of Chinese Medicine[2]Medical Department, Dongfang Hospital, Beijing University of Chinese Medicine[3]Graduate School, Shanxi University of Chinese Medicine[4]Beijing University of Chinese Medicine Libraries[5]Shanxi Modern Chinese Medicine Engineering Laboratory, Shanxi University of Chinese Medicine
OBJECTIVE: To evaluate the efficacy of the extract from Ganjiangdazao recipe(EGR) on functional dyspepsia in rats with spleen-stomach deficiency cold pattern(SSDCP) in terms of Traditional Chinese Medicine, and to investigate its pharmacodynamics.METHODS: Sixty Sprague-Dawley rats were randomly divided into the control group, SSDCP group, low-EGR SSDCP group, high-EGR SSDCP group, probiotics group, EGR group. SSDCP model was induced by gavage with the 0 ℃ edible vinegar. The symptoms and manifestations were scored by method from the relative literature, the ecological changes in cecal microflora was analyzed by16 SrRNA high-throughput sequencing technology,gastric tissues were treated by immunohistochemistry, the levels of related biochemical components related to the gastrointestinal functions were detected by enzyme-linked immunosorbent assay and colorimetry, gastric juice was measured by pH meter, blood pressure measurement by trapping tail method, surface temperature measured by infrared thermal imaging, and the content of 6-gingerol in the serum was determined by liquid-mass chromatography before and after EGR was given.RESULTS: It was found that EGR could effectively relieve the symptoms and manifestations of the SSDCP rats(P < 0.05); the value of the relative abundance of Lactobacillus, Streptococcus, Enterococcus and Coprobacillus increased, while the value of the relative abundance of Clostridium decreased(P < 0.05) in the cecal microflora in the SSDCP rats after high-EGR administration; It was also found that EGR had no substantial effect on the related biochemical components related to the gastrointestinal functions of in the SSDCP rats; and a certain amount of 6-gingerol was detected in the serum of EGR group.CONCLUSION: The pharmacodynamic site of EGR is the intestinal tract, and the mechanism behind the effect of EGR on SSDCP rats, involves increasing the beneficial bacteria and decreasing the proinflammatory bacteria in the intestinal tract. The blood pharmacodynamics of EGR remains to be further studied in the future.
基金：
National Natural Science Foundation of China:Construction of the Qi-Blood-Body Fluid Network Based on the Dynamic Detection of Human Biological Information and Research on the Network's Mechanism of Identification(No. 81473553);National Natural Science Foundation of China:Construction of TCM Qi-Function Biological Network Based on the Body Odor and Voice and Research on the Network's Mechanism (No. 81573880);National Natural Science Foundation of China:Applied biological basis of accurate measurement of Qi deficiency and Blood deficiency in TCM(No. 81873211)
基金编号：No. 81473553
语种：
中文
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区医学
小类|4 区全科医学与补充医学
最新[2025]版：
大类|4 区医学
小类|4 区全科医学与补充医学
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
通讯作者：
推荐引用方式(GB/T 7714)：
Gao Lujiao,Niu Xin,Niu Tingli,等.Curative efficacy of extract from Ganjiangdazao recipe on functional dyspepsia in rats[J].Journal of Traditional Chinese Medicine.2020,40(04):654-663.
