Ischemia-reperfusion (I/R) injury is a significant clinical problem impacting the heart and other organs, such as the kidneys and liver. This study explores the protective effects of oxycodone on myocardial I/R injury and its underlying mechanisms. Using a myocardial I/R model in Sprague-Dawley (SD) rats and an oxygen-glucose deprivation/reoxygenation (OGD/R) model in H9c2 cells, we administered oxycodone and inhibited AMP-activated protein kinase (AMPK) with Compound C (C.C). Our results showed that oxycodone significantly reduced lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) production while stabilizing mitochondrial membrane potential (MMP). Western blot and RT-qPCR analyzes confirmed that oxycodone enhances AMPK phosphorylation and upregulates the expression of Silent Information Regulator 1 (SIRT1) and Peroxisome Proliferator-Activated Receptor gamma Coactivator 1 alpha (PGC-1 alpha), thereby protecting myocardial cells. These findings suggest that oxycodone exerts significant protective effects against I/R injury by activating the AMPK pathway, offering new potential therapeutic targets for myocardial protection.
第一作者机构：[1]Peoples Hosp Jiawang Dist Xuzhou City, Xuzhou, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Jiang Yongzheng,He Hua,Jia Xinwei.Protective Role of Oxycodone in Myocardial Oxidative Stress and Mitochondrial Dysfunction Induced by Ischemia-Reperfusion[J].JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY.2025,39(2):doi:10.1002/jbt.70151.
APA：
Jiang, Yongzheng,He, Hua&Jia, Xinwei.(2025).Protective Role of Oxycodone in Myocardial Oxidative Stress and Mitochondrial Dysfunction Induced by Ischemia-Reperfusion.JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,39,(2)
MLA：
Jiang, Yongzheng,et al."Protective Role of Oxycodone in Myocardial Oxidative Stress and Mitochondrial Dysfunction Induced by Ischemia-Reperfusion".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 39..2(2025)
