Polysaccharides are the most abundant natural biopolymers on the earth and are widely used in food, medicine, materials, cosmetics, and other fields. The physicochemical properties of polysaccharides such as particle size and molecular weight often affect their practical applications. In recent years, asymmetrical flow field-flow fractionation (AF4) has been widely used in the separation and characterization of polysaccharides because it has no stationary phases or packing materials, which reduces the risk of shear degradation of polysaccharides. In this review, the principle of AF4 was introduced briefly. The operation conditions of AF4 for the analysis of polysaccharides were discussed. The applications of AF4 for the separation and characterization of polysaccharides from different sources (plants, animals, and microorganisms) over the last decade were critically reviewed. (C) 2020 Elsevier B.V. All rights reserved.
基金：
Nature Science Foundation of Hebei Province [B2016201002]; Key Project of Hebei Education Department [ZD2019009]; Medical Science Foundation of Hebei University [2020A08]; Postgraduate's Innovation Fund Project [hbu2020ss056]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|2 区化学
小类|2 区分析化学2 区生化研究方法
最新[2025]版：
大类|2 区化学
小类|1 区生化研究方法2 区分析化学
JCR分区：
出版当年[2021]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q1BIOCHEMICAL RESEARCH METHODSQ1CHEMISTRY, ANALYTICAL
第一作者机构：[1]Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China[2]Hebei Univ, Affiliated Hosp, Baoding 071000, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Xue,Zhang Wenhui,Dou Yuwei,et al.Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review[J].JOURNAL OF CHROMATOGRAPHY A.2021,1635:doi:10.1016/j.chroma.2020.461726.
APA：
Chen, Xue,Zhang, Wenhui,Dou, Yuwei,Song, Tiange,Shen, Shigang&Dou, Haiyang.(2021).Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review.JOURNAL OF CHROMATOGRAPHY A,1635,
MLA：
Chen, Xue,et al."Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review".JOURNAL OF CHROMATOGRAPHY A 1635.(2021)
化学