Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation
Oxidation is an essential biological process for human life. In this study, low density lipoprotein-Tremella fuciformis polysaccharide (LDL-TFP) complexes were prepared by electrostatic and covalent methods. The effects of preparation method on the structure and antioxidant activity of LDL-TFP complexes were investigated by asymmetrical flow field-flow fractionation (AF4) coupled with ultraviolet-visible (UV/Vis), multiangle light scattering (MALS), and differential refractive index (dRI) detectors. The results showed that the electrostatic LDLTFP complexes had a spherical structure, while the covalent LDL-TFP complexes had a rod-like structure as indicated by the ratio of R-g (radius of gyration) to Rh (hydrodynamic radius). Moreover, the results revealed that the antioxidant activity of the LDL-TFP complexes on the HepG2 could be related to the structure of LDL-TFP complexes. The antioxidant activity of LDL-TFP complexes formed by LDL modified with phospholipase A(2) was further enhanced. This study would help expand the application of TFP.
基金：
National Key Research and Development Program of China [2018YFD0400200]; Natural Science Foundation of Hebei Province [H2020201295]; Key Project of Hebei Education Department [ZD2019009]; Foun-dation of Hebei University [DXK202115]
第一作者机构：[1]Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China[2]Hebei Univ, Affiliated Hosp, Baoding, Peoples R China[*1]Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of BasicMedical Sciences, Hebei University, Baoding 071000, China
推荐引用方式(GB/T 7714)：
Chen Xue,Tian Yiling,Zhang Jie,et al.Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation[J].FOOD CHEMISTRY.2022,384:doi:10.1016/j.foodchem.2022.132619.
APA：
Chen, Xue,Tian, Yiling,Zhang, Jie,Li, Yueqiu,Zhang, Wenhui...&Dou, Haiyang.(2022).Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation.FOOD CHEMISTRY,384,
MLA：
Chen, Xue,et al."Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation".FOOD CHEMISTRY 384.(2022)
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