机构：[1]Faculty of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.[2]Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.[3]School of Clinical Medicine, Hebei University, Baoding 071000, PR China.[4]Department of Neurosurgery,Affiliated Hospital of Hebei University,Baoding 071000,PR China.河北大学附属医院重点学科神经外科[5]Faculty of Acupuncture-Moxibustion and Tuina, Hebei University of Chinese Medicine, Shijiazhuang 050200, PR China.[6]Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, PR China.[7]Endoscope Room, Department of General Surgery, Cangzhou Central Hospital, Cangzhou 061001, PR China.[8]Departments of Breast Surgery,Affiliated Hospital of Hebei University,Baoding 071000,PR China.医疗乳腺外科河北大学附属医院[9]Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Hebei Medical University, Shijiazhuang 050017, PR China.
Circular RNAs (circRNAs) have a critical regulatory function in human glioma. However, novel circRNAs related to different pathological grades of glioma and their crucial potential function are worth screening and prediction. CircRNA expression profiling was performed for 6 paired high- and low-grade glioma tissues and 5 adjacent normal brain tissues through next-generation sequencing. Quantitative real-time PCR (qRT-PCR) was conducted to validate circRNA expression. Bioinformatics analysis was performed, and circRNA-miRNA-mRNA networks were constructed. The expression and survival data of miRNAs and target genes were examined by GEPIA, Chinese Glioma Genome Atlas (CGGA), ONCOMINE, and cBioPortal databases. The RNA binding proteins (RBPs), open reading frames (ORFs) and N6-methyladenosine (m6A) modifications of the identified circRNAs were also predicted. Through multilevel research screening, 4 circRNAs (hsa_circ_0000915, hsa_circ_0127664, hsa_circ_0008362, and hsa_circ_0001467) were associated with glioma of different pathological grades and could be preferred candidates for subsequent functional analysis. Therefore, circRNAs are associated with the different pathological grades of glioma and reveal their potential critical regulatory function. CircRNAs might provide vital molecular biomarkers and potential therapeutic targets for glioma.
基金：
This work was financially supported by grants from National Natural Science Foundation of China (no. 81670273), Natural Science Foundation of Hebei province (no. H2020201050), Government-funded Provincial Clinical Medicine Talent Programs in 2017 (no. 3010601198073), Science and Technology Capacity Improvement Projects of Hebei University of Chinese Medicine in 2019 (no. KTZ2019019), Outstanding Student Scientific Research Ability Improvement Projects of Hebei University of Chinese Medicine in 2019 (no. YXZ2019002), and Graduate Innovative Ability Training Projects of Hebei Province in 2020 (no. XCXZZBS2020002) (no. hbu2020bs003)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|2 区医学
小类|2 区老年医学3 区细胞生物学
最新[2025]版：
无
第一作者：
第一作者机构：[1]Faculty of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.[2]Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.
共同第一作者：
通讯作者：
通讯机构：[1]Faculty of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.[2]Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang 050091, PR China.[3]School of Clinical Medicine, Hebei University, Baoding 071000, PR China.
推荐引用方式(GB/T 7714)：
Geng Xiuchao,Zhang Yuhao,Li Qiang,et al.Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades[J].Aging.2020,12:doi:10.18632/aging.202192.
APA：
Geng Xiuchao,Zhang Yuhao,Li Qiang,Xi Wang,Yu Wentao...&Wang Hong.(2020).Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades.Aging,12,
MLA：
Geng Xiuchao,et al."Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades".Aging 12.(2020)
