The aim of this study was to investigate the advantages of DTI, 3D-FSPGR, and BOLD-fMRI combined with neuronavigation for precise tumor resection in brain functional areas. 40 patients with intracranial tumors were retrospectively selected and divided into two groups according to their wishes. The study group (S) were performed scanning with three sequences (DTI, 3D-FSPGR, and BOLD-fMRI) combined with neuronavigation guided fusion imaging for intracranial tumor resection according to intraoperative imaging suggestions. The control group (C) were only performed conventional enhanced MRI and resected the intracranial mass using the conventional manner. All the operations were performed by the same surgeon. T-test and chi-square test were used to compare the operation time, rate of surgical resection, and KPS scores before and after surgery. Group S had less operation time while higher resection rate and better postoperative effect than Group C. DTI, 3D-FSPGR, and BOLD-fMRI combined with neuronavigation can better locate the tumor in the intracranial functional area and improve the accuracy of intracranial resection, thus improving patients' quality of life.