Objective: In order to obtain the best treatment program for Knee Osteoarthritis (KOA), to alleviate the pain of patients with KOA, and to systematically evaluate the efficacy of acupuncture cupping therapy for KOA. Methods: we used computer to search the databases of CNKI (1989-2018.10), Wanfang (1989-2018.10), VIP (1989-2018.10), PubMed (1966-2018.10), EMbase (1986-2018.10) and Cochrane Library (the 3rd issue of 2018). And the literature quality was evaluated by Jadad scale. Results: A total of 19 eligible RCT studies were included, and a total of 2,088 patients participated in the eligible clinical study. Meta-analysis results showed that the total effective rate OR combination =2.85[OR =3.98, 95%CI (2.98,5.32), P < 0.00001]. The results showed that the curative effect of acupuncture and collaterals cupping in the treatment of KOA was superior to other therapies. Conclusion: Acupuncture and cupping therapy of traditional Chinese medicine is effective and safe in treating KOA. Due to the impact of the original study on Meta-analysis results, more large samples and high-quality clinical trials are still needed to verify.