A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K(d)/H-2D(d)) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.
第一作者机构：[1]HeBei Univ, Affiliated Hosp, Baoding City, Hebei Province, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Hui,Gao Xinping,Yang Yongan,et al.New construction of an animal model for the orthotopic transplantation of an ovarian tumor[J].JOURNAL OF OVARIAN RESEARCH.2014,7:doi:10.1186/1757-2215-7-64.
APA：
Zhang, Hui,Gao, Xinping,Yang, Yongan,Wang, Weiming,Liu, Jin...&Ma, Youju.(2014).New construction of an animal model for the orthotopic transplantation of an ovarian tumor.JOURNAL OF OVARIAN RESEARCH,7,
MLA：
Zhang, Hui,et al."New construction of an animal model for the orthotopic transplantation of an ovarian tumor".JOURNAL OF OVARIAN RESEARCH 7.(2014)
