机构：[1]Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, China河北大学附属医院[2]Department of Orthopedics, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China河北医科大学第四医院
Objective: To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells. Methods: The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. The targeting relationship between miR-143-3p and MAPK7 was predicted by TargetScan and verified by dual luciferase reporter assay. MTT and Transwell assays were used to detect cell viability, migrated cells and invaded cells of U2OS cells. Results: Compared with hFOB1.19 cells, the expression of miR-143-3p was down-regulated and MAPK7 was up-regulated in U2OS cells. Cell viability, migration and invasion ability significantly decreased induced by miR-143-3p overexpression or MAPK7 knockdown in U2OS cells. The results of dual luciferase reporter assay indicated that miR-143-3p interacted with MAPK7. Furthermore, overexpression of MAPK7 could reverse the inhibitory effects on cell proliferation, migration and invasion in U2OS cells induced by miR-143-3p mimics. Conclusion: miR-143-3p could inhibit proliferation, migration and invasion of U2OS cells by targeting MAPK7.
基金：
Natural Science
Foundation of Hebei Province [H2015206314] and the Key Project of
Medical-Science Research of Hebei Province [20160159].
第一作者机构：[1]Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, China
通讯作者：
通讯机构：[1]Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding, Hebei, China[*1]Department of Orthopedics, Affiliated Hospital of Hebei University, 212 Dongfeng East Road, Baoding 071000, Hebei, China
推荐引用方式(GB/T 7714)：
Hou Yu,Feng Helin,Jiao Jianbao,et al.Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7[J].ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY.2019,47(1):2065-2071.doi:10.1080/21691401.2019.1620252.
APA：
Hou, Yu,Feng, Helin,Jiao, Jianbao,Qian, Ligang,Sun, Bo...&Liang, Zhixing.(2019).Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,47,(1)
MLA：
Hou, Yu,et al."Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 47..1(2019):2065-2071
