机构:[1]Hebei Univ, Affiliated Hosp, Basic Res Key Lab Gen Surg Digital Med, Baoding 071000, Peoples R China重点实验室河北省普通外科数字医学基础研究重点实验室河北大学附属医院[2]Tsinghua Univ, Sch Clin Med, Beijing 100084, Peoples R China[3]Beijing Univ Technol, Sch Mech & Energy Engn, Beijing 100124, Peoples R China
Achilles tendon is a motor organ that is prone to tissue adhesion during its repair process after rupture. Therefore, developing motion-responsive and anti-adhesive biomaterials is an important need for the repair of Achilles tendon rupture. Here, we report an injectable lubricative hydrogel (ILH) based on hydration lubrication mechanism, which is also motion-responsive based on sol-gel reversible transmission. The lubrication performance is achieved by zwitterionic polymers as we previously proved, and the sol-gel reversible transmission is enabled by dynamic disulfide bonds. Firstly, ILH was proved to be successfully prepared and lubricated as well as sol-gel reversible via FTIR characterization, rheological measurement and tribological tests. Then, in vitro cell experiments and coagulation tests demonstrated the optimal cytocompatibility and hemocompatibility of ILH. To evaluate the potential of ILH's biofunction in vivo, SD rats' Achilles tendon rupture & repair model was established. The animal experiments' results showed that ILH significantly prevented tendon adhesion and thus promote tendon healing by inhibiting TGF(31-Smad2/3 pathway. We believe this work will open a new horizon for tendon adhesion-free repair.
基金:
Beijing Natural Science Foundation [2244086]; Hebei Natural Science Foundation [H2022201064]; Hebei Provincial Government Subsidized Training Program for Excellent Talents in Clinical Medicine [ZF2024215]; State Key Laboratory of Oral Diseases Research Open Project Funds [SKLOD2023OF03]; Tribology Science Fund of State Key Laboratory of Tribology in Advanced Equipment [SKLTKF22B01]
第一作者机构:[1]Hebei Univ, Affiliated Hosp, Basic Res Key Lab Gen Surg Digital Med, Baoding 071000, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Hebei Univ, Affiliated Hosp, Basic Res Key Lab Gen Surg Digital Med, Baoding 071000, Peoples R China[2]Tsinghua Univ, Sch Clin Med, Beijing 100084, Peoples R China[3]Beijing Univ Technol, Sch Mech & Energy Engn, Beijing 100124, Peoples R China[*1]Basic Research Key Laboratory of General Surgery for Digital Medicine, Affiliated Hospital of Hebei University, Baoding, 071000, China