LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit
机构：[1]Hebei Univ Chinese Med, Hebei Prov Hosp Chinese Med, Dept Radiotherapy, Shijiazhuang, Hebei, Peoples R China[2]Chongqing Gen Hosp, Dept Gen Surg, Chongqing, Peoples R China[3]Key Lab Integrated Chinese & Western Med Gastroen, Shijiazhuang, Hebei, Peoples R China
Introduction: Collective cancer cell migration (CCCM) and epithelial-to-mesenchymal transition (EMT) play key roles in metastasis. This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT. Methods: Hematoxylin and eosin staining, scanning electron microscopy, transmission electron microscopy, and western blot analysis were performed. Results: LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties, including several layers of cells arranged around a central lumen, apical-basal polarity, and types of cell-cell junctions. Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT, a spheroid changing phenotype to form a monolayer high-confluent patch without lumen, without polarity. Spontaneous CCCM occurred in spheroids. Flat EMT cells adhered to the base of a dish, exhibited persistent movement as a cluster of cells, and then shed, resulting in a cluster. All cells from one cluster undergoing CCCM died. Otherwise, all cells undergoing EMT disappeared and almost all cells located in the cell reservoir survived and proliferated. Conclusion: LIM1863 is an excellent cell line to study CCCM and EMT. The group of heterogeneous cells undergoing CCCM behaves like a supracellular unit.
基金：
Hebei Province Key Research and Development Program [19277770D]; Natural Science Foundation of Hebei Province [H2018423026]; Foundation of Health and Family Planning Commission of Hebei [20180686, 20180688]; Fund of Hebei Administration of Traditional Chinese Medicine [2023020]
第一作者机构：[1]Hebei Univ Chinese Med, Hebei Prov Hosp Chinese Med, Dept Radiotherapy, Shijiazhuang, Hebei, Peoples R China[2]Chongqing Gen Hosp, Dept Gen Surg, Chongqing, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ Chinese Med, Hebei Prov Hosp Chinese Med, Dept Radiotherapy, Shijiazhuang, Hebei, Peoples R China[3]Key Lab Integrated Chinese & Western Med Gastroen, Shijiazhuang, Hebei, Peoples R China
推荐引用方式(GB/T 7714)：
Wu Jinsong,Zhi Zheng,Xu Wenzhong,et al.LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit[J].BIOCELL.2023,47(12):2671-2680.doi:10.32604/biocell.2023.043494.
APA：
Wu, Jinsong,Zhi, Zheng,Xu, Wenzhong,Li, Diancgeng,Li, Qiubo...&Liang, Xi.(2023).LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit.BIOCELL,47,(12)
MLA：
Wu, Jinsong,et al."LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit".BIOCELL 47..12(2023):2671-2680
