Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway
OBJECTIVE: To investigate the efficacy of scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen, on neurobehavior in young rats with cerebral palsy based on Notch signaling pathway.METHODS: Thirty 7-day-old rats were randomly divided into sham, model , acupuncture, 10 rats in each group. The cerebral palsy model was established by the accepted modeling method, the acupuncture group selected "Baihui (GV20)", "Sishencong (EX-HN1)", "Zhisanzhen" and "Niesanzhen" for intervention 24 h after the model was made. The body masses were recorded before and after the treatment, respectively. After the intervention, the rats were subjected to suspension experiment, slope experiment, tactile stimulation experiment and Morris water maze experiment. After the end of the experiment, the morphological changes of hippocampal histology were observed by hematoxylineosin (HE) staining under light microscope , the expression of Notch1, Notch3 , Hes5 were detected by Western blot and quantitative real-time polymerase chain reaction (PCR). RESULTS: The changes in body mass of the rats in each group were different; in behavioral experiments, compared with the sham, the suspension time of the model was shortened, the slope experiment, tactile stimulation experiment, and escape latency time were prolonged, and the number of platform crossing was reduced in the model, compared with the model, the suspension time of the acupuncture was prolonged, the slope experiment, tactile stimulation experiment, and escape latency time were shortened, and the number of platform crossing times was increased; HE staining showed severe hippocampal damage in the model and reduced hippocampal damage in the acupuncture. Western Blot and real-time fluorescence quantitative PCR showed that the expression of Notch1, Notch3 and Hes5 were increased in the model and the expression of Notch1, Notch3, Hes5 in acupuncture were decreased. CONCLUSIONS: Scalp acupuncture Yikang therapy may improve neurobehavior and reduce brain injury in rats with cerebral palsy by downregulating the expression of Notch1, Notch3, and Hes5.(c) 2023 JTCM. All rights reserved.
基金：
Key Projects of Scientific Research in Higher Education Institutions in Hebei Province [ZD2020144]; Doctoral Research Project of Hebei University of Chinese Medicine [BSZ2020002]
第一作者机构：[1]Hebei Univ Chinese Med, Dept Acupuncture & Message, Shijiazhuang 050091, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ Chinese Med, Dept Acupuncture & Message, Shijiazhuang 050091, Peoples R China[*1]epartment of Acupuncture and Message, Hebei University of Chinese Medicine, Shijiazhuang 050091, China
推荐引用方式(GB/T 7714)：
Xue Jianyi,Xu Jinyan,Huang Mao,et al.Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway[J].JOURNAL OF TRADITIONAL CHINESE MEDICINE.2023,43(2):337-342.doi:10.19852/j.cnki.jtcm.20221206.002.
APA：
Xue, Jianyi,Xu, Jinyan,Huang, Mao,Yu, Wentao,Yan, Yihui...&Yan, Siyang.(2023).Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway.JOURNAL OF TRADITIONAL CHINESE MEDICINE,43,(2)
MLA：
Xue, Jianyi,et al."Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway".JOURNAL OF TRADITIONAL CHINESE MEDICINE 43..2(2023):337-342
