Ovarian cancer was one of the most common malignant tumors in female reproductive organs. Moreover, epithelial ovarian cancer showed the highest mortality rate in gynecological tumors, posing serious threats to women's life and health. Transient Receptor Potential Cation Channel Subfamily V Member 2 (TRPV2) and matrix metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors, but there were few studies exploring the relationships of TRPV2 and MMP-2 in OC. Therefore, we evaluated the expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC patients. Immunohistochemistry was used to analyze the expressions of TRPV2 and MMP-2 in cancer tissues (N = 70) and adjacent normal tissues (N = 70) of OC patients, and the correlation of TRPV2 and MMP-2 with the occurrence and development of OC was analyzed with the combination of clinicopathological parameters of OC patients. The results showed that the expressions of TRPV2 and MMP-2 in OC tissues were significantly higher than those in adjacent normal tissues, and there were significant differences in TRPV2 and MMP-2 expressions in terms of tumor stage, differentiation, and lymph node metastasis of OC. Taken together, our results showed that protein expressions of TRPV2 and MMP-2 were closely related associated with the occurrence and development of OC.