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Telomerase reverse transcriptase suppression inhibits cell proliferation and promotes cell apoptosis in hepatocellular cancer

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机构： [1]Hebei Univ Engn, Dept Oncol 2, Affiliated Hosp, Handan, Hebei, Peoples R China [2]Hebei Univ Engn, Coll Med, Dept Pathol, 83 Congtai Rd, Handan 056002, Hebei, Peoples R China

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关键词： hepatocellular cancer telomerase reverse transcriptase proliferation cell apoptosis

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We aimed to investigate the role of telomerase reverse transcriptase (TERT) in hepatocellular cancer (HCC) cells. R software was used for differential expressed gene analysis. Western blot and quantitative real time polymerase chain reaction (qRT-PCR), respectively, were used to detect protein expression and mRNA level of TERT in tumor cell lines. Real-time quantitative telomeric repeat amplification protocol assay, MTT assay, colony formation assay, and flow cytometry (FCM) assay were used to analyze the telomerase activity, viability, proliferation, cell cycle progression and apoptosis of HCC cells. The proliferation ratio of HCC cells transfected with TERT-siRNA was significantly decreased compared with control group. Plate clone results suggested that the number of colonies also decreased in TERT-siRNA group. FCM results showed that more cells were arrested in G0/G1 phase and apoptosis rate increased in TERT-siRNA group compared with control group. TERT suppression inhibited cell proliferation but promoted cell cycle arrest and cell apoptosis. (c) 2018 IUBMB Life, 70(7):642-648, 2018

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出版当年[2019]版：

大类 | 3 区生物

小类 | 3 区生化与分子生物学 4 区细胞生物学

最新[2025]版：

大类 | 3 区生物学

小类 | 3 区生化与分子生物学 4 区细胞生物学

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出版当年[2018]版：

Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Q3 CELL BIOLOGY

最新[2023]版：

Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Q2 CELL BIOLOGY

影响因子： 3.7 最新[2023版] 4.3 最新五年平均 3.051 出版当年[2018版] 3.39 出版当年五年平均 3.236 出版前一年[2017版] 3.244 出版后一年[2019版]

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第一作者机构： [1]Hebei Univ Engn, Dept Oncol 2, Affiliated Hosp, Handan, Hebei, Peoples R China

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