Marein protects human nucleus pulposus cells against high glucose-induced injury and extracellular matrix degradation at least partly by inhibition of ROS/NF-κB pathway
Intervertebral disc degeneration (IDD), a major cause of discogenic low back pain, is a musculoskeletal disorder involving the apoptosis of nucleus pulposus cells (NPCs) and extracellular matrix (ECM) degradation. Marein is a major active flavonoid ingredient extracted from the hypoglycemic plant Coreopsis tinctoria with several beneficial biological activities including anti-diabetic effects. Nevertheless, there are no reports concerning the effects of marein on IDD. Our study aimed to evaluate the effects of marein on high glucose (HG)-induced injury and ECM degradation in human NPCs (HNPCs). CCK-8 assay was applied to evaluate cell viability. Flow cytometry analysis, a cell death detection ELISA, and caspase-3 activity assay were used to assess apoptosis. The mRNA expression of ECM-related proteins matrix metalloproteinase (MMP)-3, MMP-13, Collagen II, and aggrecan were determined by qRT-PCR. The changes of the nuclear factor-kappa B (NF-kappa B) pathway were examined by western blot. Stimulation with HG significantly reduced cell viability and induced apoptosis in HNPCs. Moreover, HG exposure increased MMP-3 and MMP-13 expression and decreased Collagen II and aggrecan expression in HNPCs. Notably, marein effectively alleviated HG-induced viability reduction, apoptosis and ECM degradation in HNPCs. We also found that marein inhibited HG-induced ROS generation and NF-kappa B activation in HNPCs. Inhibition of NF-kappa B pathway reinforced HG-induced injury and ECM degradation in HNPCs. In summary, marein protected HNPCs against HG -induced injury and ECM degradation at least partly by inhibiting the ROS/NF-kappa B pathway.
基金：
National Natural Science Foundation of China [31701238]
第一作者机构：[1]Baoding 1 Cent Hosp, Dept Endocrinol, 320 Changcheng North St, Baoding 071000, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Baoding 1 Cent Hosp, Dept Endocrinol, 320 Changcheng North St, Baoding 071000, Peoples R China
推荐引用方式(GB/T 7714)：
Yao Mingyan,Zhang Jing,Li Zhihong,et al.Marein protects human nucleus pulposus cells against high glucose-induced injury and extracellular matrix degradation at least partly by inhibition of ROS/NF-κB pathway[J].INTERNATIONAL IMMUNOPHARMACOLOGY.2020,80:doi:10.1016/j.intimp.2019.106126.
APA：
Yao, Mingyan,Zhang, Jing,Li, Zhihong,Guo, Shuqin,Zhou, Xue&Zhang, Wenjing.(2020).Marein protects human nucleus pulposus cells against high glucose-induced injury and extracellular matrix degradation at least partly by inhibition of ROS/NF-κB pathway.INTERNATIONAL IMMUNOPHARMACOLOGY,80,
MLA：
Yao, Mingyan,et al."Marein protects human nucleus pulposus cells against high glucose-induced injury and extracellular matrix degradation at least partly by inhibition of ROS/NF-κB pathway".INTERNATIONAL IMMUNOPHARMACOLOGY 80.(2020)
