Caveolin-1 is an important gene in breast cancer. Based on the analysis of cellular and molecular levels. Further discussions on the regulation of phosphorylation of Her-2 Caveolin-1 (Cav-1) and roles in the Akt/ERK pathway have been made. We selected 50 cases of invasive breast cancer tissues and used immunohistochemical techniques to assess cav-1, and to understand its relationship with EGFR, Her-2, Ki-67 expression as well as with other conventional clinico-pathological parameters. Ectopic expression of cav-1 in human breast cancer cell lines was performed to investigate changes in cellular proliferation, migration. The role of cav-1 in p-Her-2 expression and signaling was evaluated by Western blot. The expression of Cav-1 is associated with Cav-1 and Her-2/p-Her-2 and malignance in the breast tissue. The expression of Cav-1 is associated with lymph node metastasis and clinical stage. Cav-1 expression at higher levels of human breast cancer cell proliferation, migration and invasion ability has changed, with the increase of EGF stimulation. Cav-1 had no obvious effect on total expression of Her-2, but can regulate the phosphorylation level of Her-2, under the EGF stimulation; it can decline activation levels of Akt/ERK pathway. Cav-1 may promote the growth of breast cancer cells via enhancing EGF-induced Her-2 activation (phosphorylation) and downstream signal transduction.